Computer Simulation of Radial Immunodiffusion
نویسندگان
چکیده
منابع مشابه
Silver-enhanced radial immunodiffusion assay
Silver-staining of immunoprecipitates extends the sensitivity of the radial immunodiffusion assay by tenfold. This modification permits the quantification of apolipoproteins A-1, A-11, C, and E at levels of 0.2-1.0 mg/d in plasma samples at a sensitivity threshold of 10 ng. The silver-enhanced radial immunodiffusion method is readily adapted from the standard method, simple and inexpensive to p...
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In descriptions of the quantitative estimation of proteins by radial immunodiffusion some authors read the results while the ring is still growing, others wait until equilibrium has been reached. While assembling the material for a review (Grant and Butt, 1970), I was struck by the absence of clear statements on the principles involved in these two different approaches and the consequent possib...
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A simple method is described for quantitative determination of lgG, IgA, 1gM, a2M, and other proteins without dilution of the sample. Strips of gelatinized cellulose acetate spread with antiserum are used as a supporting medium. The method is accurate, highly reproducible, and seems particularly suitable for routine work because it permits determination of hypo-, normal, and hyperglobulinemia o...
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Platelet-associated IgG (PAIgG) was measured by a simple radial immunodiffusion technique using washed solubilized platelets and commercially available immunoplates. Subjects with normal platelet counts had PAIgG levels of 1.5--7.0 fg/platelet. Subjects with idiopathic immune thrombocytopenic purpura (ITP) had levels ranging from 5.7 to 70.5 fg/platelet. All patients with recurrent ITP and 85% ...
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A direct system for screening large numbers of staphylococcal isolates for enterotoxin production has been developed. The system employs polyvalent (serotypes A, B, C, D, and E) immunodiffusion assay slides in conjunction with a multiple-culturing system for toxin production. With the combined system, as many as 50 cultures can be screened simultaneously on a single assay slide having a sensiti...
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ژورنال
عنوان ژورنال: Biophysical Journal
سال: 1973
ISSN: 0006-3495
DOI: 10.1016/s0006-3495(73)85996-x